Vacuum Infiltration Based Agrobacterium Mediated Gene Transfer to Lentil (Lens CulinarisM.) Tissues
نویسندگان
چکیده
منابع مشابه
Genetic Transformation of Oat Mediated by Agrobacterium is enhanced with Sonication and Vacuum Infiltration
Background: Oat (Avena sativa) with high nutritive value and fiber content is used as the main food grain in many countries for human diet as well as animal feed. Recently, it became difficult to transfer new genes through the conventional breeding due to the lack of desirable traits. Objectives: The current study aimed at achieving a st...
متن کاملSonication and Vacuum Infiltration Enhanced Agrobacterium Rhizogenes Mediated Transformation in Soybean
Objective: This study involved the formulation of protocol of Agrobacterium rhizogenes mediated transformation for the detailed study of isoflavones (IFs) metabolism in soybean. Methods: Cotyledons were separated from 4-day-old soybean seedlings and infected with three different A. rhizogenes strains under various time durations of sonication, vacuum infiltration and cocultivated on Murashige a...
متن کاملOptimization of gene transfer with Agrobacterium to Bonab tomato cultivar using chitinase gene
Tomato is an important economic crop with nutritional and scientific value for study. Due to its agronomic importance and sensitivity to biotic and abiotic stresses, using new methods in breeding this crop is necessary. In this study, the effects of some factors, such as pre-cultivation time (1, 2, 3 and 4 days), Agrobacterium concentration (OD600nm = 0.4, 0.6, 0.8, and 1), inoculation time (5,...
متن کاملCompetence of Immature Maize Embryos for Agrobacterium-Mediated Gene Transfer.
Agrobacterium-mediated transfer of viral sequences to plant cells (agroinfection) was applied to study the susceptibility of immature maize embryos to the pathogen. The shoot apical meristem of immature embryos 10 to 20 days after pollination from four different maize genotypes was investigated for competence for agroinfection. There was a direct correlation between different morphological stag...
متن کاملHorizontal gene transfer from Agrobacterium to plants
Most genetic engineering of plants uses Agrobacterium mediated transformation to introduce novel gene content. In nature, insertion of T-DNA in the plant genome and its subsequent transfer via sexual reproduction has been shown in several species in the genera Nicotiana and Linaria. In these natural examples of horizontal gene transfer from Agrobacterium to plants, the T-DNA donor is assumed to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2002
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2002.10819151